Praesto RoboColumns® are designed for easy process development and parameter screening, as well as for small scale purification or sample preparation. Pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, offering both very high reproducibility and very good scalability. The RoboColumn® format is designed for use with robotic workstations for HTPD work. Praesto RoboColumns® are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto SP45 is an agarose based strong cation exchange resin designed for both large scale biomolecule purification and small scale applications. Praesto SP45 can be used for purification of different biomolecules, including proteins, peptides and oligonucleotides. The particle size (45µm), makes Praesto SP45 the smallest particle of the Praesto family and thus particular useful for high resolution applications.


Principal Applications

  • Protein purification
  • MAb Purification


  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • One Row of 8 prepacked 200µl columns

Typical Physical & Chemical Characteristics:

Polymer Structure Agarose - Strong Cation Exchanger
Appearance Spherical beads
Functional Group Sulfopropyl
Inner diameter 5 mm
Packed bed height 10 mm
Column volume 200 µl
Connector for use with liquid robotic handling stations
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Halogenated organic solvents, hexane, oxidising agents, anionic detergents
Working pressure (max.) 8 bar
Ionic capacity - medium 0.11 - 0.16 mmol H⁺/ml
Ionic capacity - resin 0.11 - 0.16 mmol/ml
Particle Size - µm 45 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 80 lgG/ml
Dynamic Binding Capacity (min.)- 3 minutes residence time 80 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 200 cm/h
pH stability, CIP (short term) 3 - 14
pH stability, working range 4 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol
Recommended Storage 0.2M sodium acetate