Praesto MiniChrom columns are designed for process development and parameter screening, as well as for small scale purification or sample preparation. MiniChrom columns are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto MiniChrom pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, as well as convenience in media screening and easy, direct connection to most chromatography systems. Praesto Q90 is an agarose-based strong anion exchange resin, designed for large scale biomolecule purification, including proteins, peptides and oligonucleotides. The relatively large particle size (90 µm) makes Praesto Q90 ideal for capture or intermediate purification of proteins when large sample volumes needs to be processed within a limited time frame.

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Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Prepacked 5ml chromatography column

Typical Physical & Chemical Characteristics:

Polymer Structure Agarose - Strong Anion Exchanger
Appearance Spherical beads
Functional Group Quaternary amine
Inner diameter 8 mm
Packed bed height 100 mm
Column volume 5 ml
Connector fingertight 1/16", female
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Halogenated organic solvents, hexane, oxidising agents, anionic detergents
Working pressure (max.) 30 bar
Ionic capacity - resin 0.14 - 0.18 mmol/ml
Ionic capacity - medium 0.14 - 0.18 mmol Cl⁻/ml
Particle Size - µm 90 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 50 BSA/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 800 cm/h
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol