Praesto HT columns are designed for efficient resin screening, as well as for optimization and verification. HT Columns from Purolite Life Sciences streamline your separations, and are available pre-packed with any Praesto Affinity or Ion Exchange Chromatography agarose resin.

Columns are available in 1 ml and 5 ml bed volumes, with a 2.5 cm bed height. HT Columns are compatible with all commonly-used chromatography systems due to universal 1/16" connectors. HT Columns are constructed of polypropylene, preventing interaction with biological molecules and ensuring biocompatibility.

Praesto Q90 is an agarose-based strong anion exchange resin, designed for large scale biomolecule purification, including proteins, peptides and oligonucleotides. The relatively large particle size (90 µm), makes Praesto Q90 ideal for capture or intermediate purification of proteins when large sample volumes needs to be processed within a limited time frame.

CONTACT A SPECIALIST
 

Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Prepacked 1ml column

Typical Physical & Chemical Characteristics:

Polymer Structure Agarose - Strong Anion Exchanger
Appearance Spherical beads
Functional Group Quaternary amine
Packed bed height 25 mm
Column volume 1 ml
Connector Universal 10.32 (1/16") UNF Threads
Column material Polypropylene
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Halogenated organic solvents, hexane, oxidising agents, anionic detergents
Working pressure (max.) 5 bar
Ionic capacity - resin 0.14 - 0.18 mmol/ml
Ionic capacity - medium 0.14 - 0.18 mmol Cl⁻/ml
Particle Size - µm 90 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 50 BSA/ml
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol