Praesto RoboColumns® are designed for easy process development and parameter screening, as well as for small scale purification or sample preparation. Pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, offering both very high reproducibility and very good scalability. The RoboColumn® format is designed for use with robotic workstations for HTPD work. Praesto RoboColumns® are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto Q65 is an agarose-based strong anion exchange resin. Praesto Q65 is designed for large scale biomolecule purification, including proteins, peptides and oligonucleotides. The particle size (65 µm) makes Praesto Q65 ideal for intermediate purification of proteins.

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Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • One Row of 8 prepacked 200µl columns

Typical Physical & Chemical Characteristics:

Polymer Structure Agarose - Strong Anion Exchanger
Appearance Spherical beads
Functional Group Quaternary amine
Inner diameter 5 mm
Packed bed height 10 mm
Column volume 200 µl
Connector for use with liquid robotic handling stations
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol
Avoid Halogenated organic solvents, hexane, oxidising agents, anionic detergents
Working pressure (max.) 8 bar
Ionic capacity - resin 0.14 - 0.18 mmol/ml
Ionic capacity - medium 0.14 - 0.18 mmol Cl⁻/ml
Particle Size - µm 65 µm
Dynamic Binding Capacity (min.) - 6 minutes residence time 60 BSA/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column (pressure-packed at 4 bar) 400 cm/h
pH stability, CIP (short term) 2 - 14
pH stability, working range 3 - 13
Recommended Storage 4 - 30 °C
Recommended Storage 20% ethanol