Q45 HT Column (5ml)
Highly cross linked Strong Anion Exchanger
Praesto HT columns are designed for efficient resin screening, as well as for optimization and verification. HT Columns from Purolite Life Sciences streamline your separations, and are available pre-packed with any Praesto Affinity or Ion Exchange Chromatography agarose resin.
Columns are available in 1 ml and 5 ml bed volumes with a 2.5 cm bed height. HT Columns are compatible with all commonly-used chromatography systems, due to universal 1/16" connectors. HT Columns are constructed of polypropylene, preventing interaction with biological molecules and ensuring biocompatibility.
Praesto Q45 is an agarose-based strong anion exchange resin. Praesto Q45 is designed for large-scale biomolecule purification, but is also an excellent choice for small scale applications. Praesto Q45 can be used for purification of different biomolecules, including proteins, peptides and oligonucleotides. The particle size (45µm) makes Praesto Q45 the smallest particle of the Praesto family and thus particular useful for high resolution applications.
CONTACT A SPECIALIST
|Polymer Structure||Agarose - Strong Anion Exchanger|
|Functional Group||Quaternary amine|
|Packed bed height||25 mm|
|Column volume||5 ml|
|Connector||Universal 10.32 (1/16") UNF Threads|
|Chemical stability||All commonly used aqueous buffers, pH 1-14, organic solvents, 1M NaOH, 8M urea, 6M guanidine hydrochloride, 30% isopropanol and 70% ethanol|
|Avoid||Halogenated organic solvents, hexane, oxidising agents, anionic detergents|
|Working pressure (max.)||5 bar|
|Ionic capacity - resin||0.14 - 0.18 mmol/ml|
|Ionic capacity - medium||0.14 - 0.18 mmol Cl⁻/ml|
|Particle Size - µm||45 µm|
|Dynamic Binding Capacity (min.) - 6 minutes residence time||70 BSA/ml|
|pH stability, CIP (short term)||2 - 14|
|pH stability, working range||3 - 13|
|Recommended Storage||4 - 30 °C|
|Recommended Storage||20% ethanol|