APc HT Column (5ml)
Highly cross linked agarose Protein A affinity resin
Praesto HT columns are designed for efficient resin screening, as well as for optimization and verification. HT Columns from Purolite Life Sciences streamline your separations, and are available pre-packed with any Praesto Affinity or Ion Exchange Chromatography agarose resin.
Columns are available in 1 ml and 5 ml bed volumes with a 2.5 cm bed height. HT Columns are compatible with all commonly-used chromatography systems, due to universal 1/16" connectors. HT Columns are constructed of polypropylene, preventing interaction with biological molecules and ensuring biocompatibility.
Praesto APc is a Protein A resin designed on a novel high flow agarose backbone which addresses today s early-phase clinical trial challenges for high productivity togetherwith cost effective MAb capture. It is purpose-designed and evaluated for phase I and II clinical trials, where typically less than 20 reuse cycles are performed. Alternatively Praesto APc can be implemented across all phases due to long life time. Before PIII, for innovator therapies, Purolite Life Sciences recommends you then switch from Praesto APc to Praesto AP since they both utilize the same Protein A ligand species.
CONTACT A SPECIALIST
|Polymer Structure||Highly cross linked agarose|
|Functional Group||Protein A|
|Packed bed height||25 mm|
|Column volume||5 ml|
|Connector||Universal 10.32 (1/16") UNF Threads|
|Chemical stability||All commonly used aqueous buffers, pH 1-14, organic solvents|
|Avoid||Halogenated organic solvents, hexane|
|Working pressure (max.)||5 bar|
|Particle Size - µm||85 µm|
|Dynamic Binding Capacity (min.)- 3 minutes residence time||25 hlgG/ml|
|pH stability, CIP (short term)||2 - 12|
|pH stability, working range||3 - 10|
|Recommended Storage||2 - 8 °C|
|Recommended Storage||20% ethanol|