Praesto HT columns are designed for efficient resin screening, as well as for optimization and verification. HT Columns from Purolite Life Sciences streamline your separations, and are available pre-packed with any Praesto Affinity or Ion Exchange Chromatography agarose resin.

Columns are available in 1 ml and 5 ml bed volumes with a 2.5 cm bed height. HT Columns are compatible with all commonly-used chromatography systems, due to universal 1/16" connectors. HT Columns are constructed of polypropylene, preventing interaction with biological molecules and ensuring biocompatibility.

Praesto APc is a Protein A resin designed on a novel high flow agarose backbone which addresses today s early-phase clinical trial challenges for high productivity togetherwith cost effective MAb capture. It is purpose-designed and evaluated for phase I and II clinical trials, where typically less than 20 reuse cycles are performed. Alternatively Praesto APc can be implemented across all phases due to long life time. Before PIII, for innovator therapies, Purolite Life Sciences recommends you then switch from Praesto APc to Praesto AP since they both utilize the same Protein A ligand species.

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Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Prepacked 5ml chromatography column

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Protein A
Packed bed height 25 mm
Column volume 5 ml
Connector Universal 10.32 (1/16") UNF Threads
Column material Polypropylene
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents
Avoid Halogenated organic solvents, hexane
Working pressure (max.) 5 bar
Particle Size - µm 85 µm
Dynamic Binding Capacity (min.)- 3 minutes residence time 25 hlgG/ml
pH stability, CIP (short term) 2 - 12
pH stability, working range 3 - 10
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol