Praesto HT columns are designed for efficient resin screening, as well as for optimization and verification. HT Columns from Purolite Life Sciences streamline your separations, and are available pre-packed with any Praesto Affinity or Ion Exchange Chromatography agarose resin.

Columns are available in 1 ml and 5 ml bed volumes with a 2.5 cm bed height. HT Columns are compatible with all commonly-used chromatography systems, due to universal 1/16" connectors. HT Columns are constructed of polypropylene, preventing interaction with biological molecules and ensuring biocompatibility.

Praesto AP is a Protein-A resin designed on a novel high flow agarose backbone which uses a recombinant alkaline-tolerant Protein-A ligand expressed in E.coli. The Protein-A ligand used for Praesto AP resulted from protein engineering of one of the IgG-binding domains of Protein-A. In particular, the amino acids sensitive to alkali conditions were identified and then substituted with more stable ones to deliver superior alkaline stability which provides hundreds of re-cycles. Praesto AP addresses today s late stage clinical manufacturing challenges for high productivity together with cost-efficient MAb capture. It is purpose-designed and evaluated for the production of late phase clinical trial material, PIII and commercial manufacture, where typically hundreds of reuse cycles are performed.

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Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Prepacked 5ml chromatography column

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Protein A
Packed bed height 25 mm
Column volume 5 ml
Connector Universal 10.32 (1/16") UNF Threads
Column material Polypropylene
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents
Avoid Halogenated organic solvents, hexane
Working pressure (max.) 5 bar
Particle Size - µm 85 µm
Dynamic Binding Capacity (min.)- 3 minutes residence time 25 hlgG/ml
pH stability, CIP (short term) 2 - 12
pH stability, working range 3 - 10
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol