Praesto RoboColumns® are designed for easy process development and parameter screening, as well as for small scale purification or sample preparation. Pre-packed columns provide a small bed volume for fast results and minimal sample and buffer consumption, offering both very high reproducibility and very good scalability. The RoboColumn® format is designed for use with robotic workstations for HTPD work. Praesto RoboColumns® are packed by Atoll GmbH and delivered ready for use. They are 100% quality checked for HETP and asymmetry. Individual laser labelling ensures traceability to corresponding resin and column performance data. The columns are made of biocompatible polypropylene. Praesto AC is a Protein A resin designed to address today's clinical manufacturing challenges for high productivity, cost-effective MAb capture. Praesto AC combines good capacity, excellent pressure/flow performance and NaOH CIP stability for up to 20 cycles, meeting the common requirements for production of materials for PI and PII clinical trials. Praesto AC can also be used in small scale MAb purification, purification of MAbs for diagnostics, in process development and in pre-clinical processes

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Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • One Row of 8 prepacked 200µl columns

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Protein A
Inner diameter 5 mm
Packed bed height 10 mm
Column volume 200 µl
Connector for use with liquid robotic handling stations
Column material Polypropylene
Bed support Filter
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents
Avoid Halogenated organic solvents, hexane
Working pressure (max.) 8 bar
Particle Size - µm 85 µm
Dynamic Binding Capacity (min.)- 3 minutes residence time 25 hlgG/ml
Pressure/flow (min.) - at 3 bar in a 2.6 x 20 cm column 500 cm/h
pH stability, CIP (short term) 2 - 12
pH stability, working range 3 - 10
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol