Praesto HT columns are designed for efficient resin screening, as well as for optimization and verification. HT Columns from Purolite Life Sciences streamline your separations, and are available pre-packed with any Praesto Affinity or Ion Exchange Chromatography agarose resin.

Columns are available in 1 ml and 5 ml bed volumes with a 2.5 cm bed height. HT Columns are compatible with all commonly-used chromatography systems, due to universal 1/16" connectors. HT Columns are constructed of polypropylene, preventing interaction with biological molecules and ensuring biocompatibility.

Praesto AC is a Protein A resin designed to address today's clinical manufacturing challenges for high productivity, cost-effective MAb capture. Praesto AC combines good capacity, excellent pressure/flow performance and NaOH CIP stability for up to 20 cycles, meeting the common requirements for production of materials for PI and PII clinical trials. Praesto AC can also be used in small scale MAb purification, purification of MAbs for diagnostics, in process development and in pre-clinical processes

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Principal Applications

  • Protein purification
  • MAb Purification

Advantages

  • Cost effective without compromising buffer consumption or processing time
  • Very high reproducibility and scalability
  • Pre-packed and pre-qualified
  • High productivity and high capacity
  • Excellent pressure/flow performance
  • Secure, validated supply and regulatory support

Regulatory Approvals

  • Manufactured under cGMP conditions

Typical Packaging

  • Prepacked 5ml chromatography column

Typical Physical & Chemical Characteristics:

Polymer Structure Highly cross linked agarose
Appearance Spherical beads
Functional Group Protein A
Packed bed height 25 mm
Column volume 5 ml
Connector Universal 10.32 (1/16") UNF Threads
Column material Polypropylene
Chemical stability All commonly used aqueous buffers, pH 1-14, organic solvents
Avoid Halogenated organic solvents, hexane
Working pressure (max.) 5 bar
Particle Size - µm 85 µm
Dynamic Binding Capacity (min.)- 3 minutes residence time 25 hlgG/ml
pH stability, CIP (short term) 2 - 12
pH stability, working range 3 - 10
Recommended Storage 2 - 8 °C
Recommended Storage 20% ethanol